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Showed consistently differential expression between the NC group and the NA group in all three parallel PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/2902681 experiments. A total of 24 protein spots were successfully identified using LC-MS/MS, and these spots corresponded to 20 unique proteins. Among the 24 spots, 10 were down-regulated and 11 were up-regulated in the acupuncture normal rats (Table 1). Both up- and down-regulated expression patterns were observed for the actin alpha skeletal muscle protein, which may reflect differential post-translational modification of the same protein.Bioinformatic analysis of proteins differentially expressed following acupunctureDifferentially expressed proteins after acupuncture were classified according to biological process or molecularXu et al. BMC Complementary and Alternative Medicine 2014, 14:375 http://www.biomedcentral.com/1472-6882/14/Page 4 ofTable 1 The 25 differentially expressed proteins between NC and NA ratsSpot No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24a)Protein name Alpha-1-antiproteinase Gama-enolase Calreticulin Phosphatidylethanolamine-binding protein 1 Actin, alpha skeletal muscle Actin, cytoplasmic 1 Beta-enolase Creatine kinase B-type Actin, alpha skeletal muscle serum albumin precursor Heat shock cognate 71 kDa protein Transgelin 2 Creatine kinase B-type Fibrinogen beta chain Heat shock cognate 71 kDa protein Glyceraldehyde-3-phosphate dehydrogenase 22 kDa protein Heterogeneous nuclear ribonucleoproteins A2/B1 Tubulin alpha-1A chain Phosphoglycerate mutase 1 Serum albumin precursor NG,NG-dimethylarginine dimethylaminohydrolase 2 Annexin A5 Advanced glycosylation end product-specific receptor Peroxiredoxin-Gene symbol Serpina1 Eno2 Calr Pebp1 Acta1 Actb Eno3 Ckb Acta1 Alb Hspa8 Tagln2 Ckb Fgb Hspa8 Gapdh -- Hnrnpa2b1 Tuba1a Pgam1 Alb Ddah2 Anxa5 Ager PrdxSwiss-prot No. P17475 P07323 P18418 P31044 P68136 P60711 P15429 P07335 P68136 P02770 P63018 Q5XFX0 P07335 P14480 P63018 P04797 -- A7VJC2 P68370 P25113 P02770 Q6MG60 P14668 Q63495 OTheoretical Mr (kDa)/PI 46.1/5.7 47.0/7.0 48.0/4.3 20.8/5.5 42.0/5.2 41.7/5.3 47.0/7.0 42.7/5.4 42.0/5.2 68.7/6.0 70.9/5.4 23.4/7.6 42.7/5.4 54.2/7.9 70.9/5.4 35.8/8.1 21.9/9.8 37.4/9.0 50.1/4.9 28.8/6.7 68.7/6.0 29.7/5.7 35.7/4.9 42.7/5.8 24.8/5.Pept./Cov a) 6/11.68 2/5.07 49/36.06 9/25.13 29/22.02 13/10.67 2/5.07 2/4.46 8/11.94 7/6.58 35/30.03 15/32.38 2/4.46 55/45.3 19/18.89 20/20.12 7/25.00 46/36.83 2/5.32 11/16.93 39/30.59 6/11.93 14/23.20 7/8.21 13/26.34Fold changeb) 2.62 3.2 5.78 2.51 2.49 4.17 3.29 3.18 4.58 ++ c) 2.26 ++ 2.27 ?d)3.16 3.36 2.21 3.1 17.16 ?2.5 ++ ++ 2.37 ?The number of matching peptides (Pept.) and percentage of the total amino acid sequence covered by the peptides ( Seq. Cov) from the mass mapping experiments. The change level in optical density of the protein spots in NA rats compared with NC rats. c) ++ represents spots detected in gels from the NA samples but not detected in NC samples, which was considered up-regulated in the NA group. d) ?represents spot detected in gels from the NC samples but not detected in NA samples, which was considered down-regulated in the NA group.b)function using the WebGestalt software package on the basis of hypergeometric tests as described in the Methods section. Resulting BP networks are shown as Directed Acyclic Graphs (DAG), which are color-coded for P values < 0.05 (Figure 2A). Particular enrichment was found for stability regulation and stimulation-associated proteins and proteins invol.

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